|Rose, Julie||National Oceanic and Atmospheric Administration (NOAA-Milford)||Principal Investigator|
|Hutchins, David A.||University of Southern California (USC)||Co-Principal Investigator|
|Kinkade, Danie||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in January 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0601). Water was collected at 75.00S, 177.36E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Water was prescreened through acid-washed 200μm Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 12 4.5-L and 12 2.7-L acid washed trace metal clean clear polycarbonate bottles for incubation. Half of the bottles were spiked with 1.0nM FeCl3 (final concentration) at the beginning of the experiment. Bottles were incubated in two temperature controlled deck-board incubators (Feng et al., 2009; Hare et al., 2007). Incubators were screened to 18% of Io using two layers of neutral density filter. One incubator was kept at ambient temperature (0 deg C), while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for seven days. The 4.5-L bottles were sampled daily and the 2.7-L bottles were only sampled on the final day of the experiment. All sampling occurred under a laminar flow hood using trace metal clean techniques.
Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.
Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.
Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.
Total diatom abundance and community composition was determined for samples preserved with 1% glutaraldehyde (final concentration) and stored in the dark at 4 deg C until analysis. Twenty-five mL samples were settled in Utermöhl chambers for at least 18 h and enumerated using light microscopy at 400x magnification with a Zeiss Axiovert S100 inverted microscope (Utermohl, 1958). Diatoms were identified to the genus level.
Throndsen, J. 1978. Preservation and storage. In Phytoplankton manual, ed. A. Sournia, 69-74. Paris: UNESCO.
Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.
BCO-DMO Processing Notes:
- File was sorted by treatment
- Added lat,lon values of original water sampling location to file
- Added BCO-DMO header line
- File was transposed to serve data by taxon and abundance in columns
|lat||Latitude component of geographic position where water was sampled.||decimal degrees|
|lon||Longitude component of geographic position where water was sampled.||decimal degrees|
|treatment||Experimental conditions varied during the experiment. Four treatments were used: Low temperature, low iron (LTLF); low temperature, high iron (LTHF); high temperature, low iron (HTLF); high temperature, high iron(HTHF)||dimensionless|
|day||Sampling day during experiment. The experiment was conducted during January, 2006.||dimensionless|
|bottle||Experimental bottle number.||dimensionless|
|abundance||Number of cells of each taxa per ml.||cells/ml|
|taxon||Taxanomic name of each organism counted.||dimensionless|
|Dataset-specific Instrument Name|| |
|Generic Instrument Name|| |
|Dataset-specific Description|| |
Samples were enumerated using light microscopy at 400x magnification with a Zeiss Axiovert S100 inverted microscope (Utermohl, 1958). Utermohl, H. 1958. Zur Vervollkommung der quantitativen phytoplankton-methodik. Mitteilungen der Internationalen Vereinigung fur Limnologie 9: 1-38.
|Generic Instrument Description|| |
An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana). Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives. The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications.
RVIB Nathaniel B. Palmer
|Start Date|| |
|End Date|| |
This was the first of two Controls of Ross Sea Algal Community Structure (CORSACS) project cruises and was funded by the NSF Office of Polar Programs. The NBP0601 cruise was conducted in the Ross Sea in December 2005 and January 2006, Ross Sea, ca. 65.21°S-78.65°S, 164.98°E-164.70°W, and supported by NSF research grant, OPP-0338097. The 'Science Pan and Project Description' document includes details of the cruise sampling strategy. Related Files: Science Plan and Project Descriptions (PDF file)Cruise track map (PDF file)Photo of Ice Breaker Nathaniel B. Palmer on station near Beaufort Island (JPG image) Related Sites: MGDS catalog: http://www.marine-geo.org/tools/search/entry.php?id=NBP0601
This document is created by info v 4.1f 5 Oct 2018 from the content of the BCO-DMO metadata database. 2020-08-08 08:39:15