[Directory] [Data...]

Data server object:  MUSiCC




  
    Nutrients, pigments, silicate and experimental data collected aboard the OCEANUS during cruise OC1504A in the North Pacific Ocean from 2015-04-19 to 2015-05-06
    
  
  
    
    

Nutrients, pigments, silicate and experimental data collected aboard the OCEANUS during cruise OC1504A in the North Pacific Ocean from 2015-04-19 to 2015-05-06

Website: https://www.bco-dmo.org/dataset/650831
Data Type: Cruise Results
Version: Final
Version Date: 2016-07-12

Project
» Linking physiological and molecular aspects of diatom silicification in field populations (Diatom Silicification)
ContributorsAffiliationRole
Brzezinski, Mark A.University of California-Santa Barbara (UCSB-MSI)Principal Investigator
Thamatrakoln, KimberleeRutgers University (Rutgers IMCS)Co-Principal Investigator
Ake, HannahWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager
Allison, DickyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager

Abstract
These data include nutrient, pigment, silica and experimental data collected aboard the OCEANUS during cruise OC1504A in the North Pacific Ocean along the California Coast from 2015-04-19 to 2015-05-06. The water samples were collected by CTDs. Silica production rates were characterized by delivering incremental increases in silicic acid (Si) along with a radioactive isotope of silicon (32Si). This is an extremely sensitive assay and can determine the maximum production rates of the community being studied and the degree that its growth is being limited by lack of Si. These data were collected by Mark Brzezinski from the University of California at Santa Barbara as part of Linking Physiological and Molecular Aspects of Diatom Silicification.


Coverage

Spatial Extent: N:42.654 E:-120.82 S:34.551 W:-124.482
Temporal Extent: 2015-04-20 - 2015-05-01

Dataset Description

Nutrients, pigments, bottle data, and experimental and survey biogeochemical data from cruise OC1504A. "Silica production rates were characterized by delivering incremental increases in silicic acid (Si) along with a radioactive isotope of silicon (32Si). This extremely sensitive assay can determine the maximum production rates of the community being studied and the degree that its growth is being limited by lack of Si." (from cruise blog)

CRUISE BLOG 

These data are restricted until 2017 (2 years after the cruise), or contact Mark Brzezinski, PI.


Acquisition Description

See the following protocol documents:

32Si Sample Processing (.doc)
Biogenic Si Analysis (.doc)
Dissolved Si Analysis (.doc)


Processing Description

BCO-DMO Processing Notes:
- system added negative to the longitude because West;
- modified parameter names to conform with BCO-DMO naming conventions;
- replaced ~ with 'nd' to indicate 'no data';
 


[ table of contents | back to top ]

Parameters

ParameterDescriptionUnits
cruise_idcruise during which sample was collected unitless
brief_descthe way the project refers to the cruise unitless
eventevent number from Bruland event log unitless
stationsampling station number unitless
date_utcdate at longitude 0; the universal time coordinate (UTC);format:dd-mon-yy unitless
time_utctime at longitude 0; the universal time coordinate (UTC);format:HH:MM unitless
latlatitude in decimal degrees decimal degrees
lonlongitude in decimal degrees; West is negative decimal degrees
depth_wbottom depth in meters meters
castcast type (CTD) unitless
ISO_DateTime_UTCdate and time formatted to ISO 8601 standard; added by DMO;format:YYYY-mm-ddTHH:MM:SS.xx yyyy-MM-dd'T'HH:mm:ss
depthsampling depth in meters meters
sample_noidentifier for all types of samples at the given depth unitless
bottle_rosetterosette bottle number unitless
PAR_percentpercent light level; original name: percent lo percent
bottle_pooledsample identifier denoting whether sample was pooled from several Niskins unitless
depth_targettarget depth for sample collection meters
PO4dissoved phosphate concentration in micromoles; analyzed in UCSB MSI Analytical lab;http://www.msi.ucsb.edu/services/analytical-lab/seawater-nutrients-fia micromoles per liter
NO3_NO2dissolved nitrate+nitrite concentration in micromoles; analyzed in UCSB MSI Analytical lab; http://www.msi.ucsb.edu/services/analytical-lab/seawater-nutrients-fia micromoles per liter
chl_totaltotal chlorophyll (a+b+c) in micrograms per litre determined fluorometrically micrograms per liter
chlachlorophyll a in micrograms per litre determined fluorometrically micrograms per liter
phaeophaeophytin in micrograms per litre determined fluorometrically micrograms per liter
dSisilicic acid concentration in micromoles (also known as dissolved silicon concentration or dSi) micromoles per Si per liter
bSiparticulate biogenic silica in micromoles Si per litre micromoles per Si per liter
Si_prod_4hfour hour incubation silica production rate; original name=4h-32Si rho micromoles per Si per liter per day
Si_prod_bionorm_4hfour hour incubation biomass normalized silica production rate; original name=4h-32Si Vb per day
Si_prod_Na2SiO3_4hfour hour incubation silica production rate after the addition of sodium silicate to a final concentration of ~20uM Na2SiO3; original name=4h-32Si E rho micromoles per Si per liter per day
Si_prod_bionorm_Na2SiO3_4hfour hour incubation biomass normalized silica production rate after the addition of sodium silicate to a final concentration of ~20uM Na2SiO3; original name=4h-32Si E Vb per day
Si_prod_12htwelve hour incubation silica production rate; original name=12h-32Si rho micromoles per Si per liter per day
Si_prod_bionorm_12htwelve hour incubation biomass normalized silica production rate; original name=12h-32Si Vb per day
Si_prod_Na2SiO3_12htwelve hour incubation silica production rate after the addition of sodium silicate to a final concentration of ~20uM Na2SiO3; original name=12h-32Si E rho micromoles per Si per liter per day
Si_prod_bionorm_Na2SiO3_12htwelve hour incubation biomass normalized silica production rate after the addition of sodium silicate to a final concentration of ~20uM Na2SiO3; original name=12h-32Si E Vb per day

[ table of contents | back to top ]

Deployments

OC1504A

Website
Platform
R/V Oceanus
Report
Start Date
2015-04-19
End Date
2015-05-02
Description
Data for the project "Linking physiological and molecular aspects of diatom silicification in field populations" (PIs Kimberlee Thamatrakoln and Mark Brzezinski) were collected on this cruise.


[ table of contents | back to top ]

Project Information

Linking physiological and molecular aspects of diatom silicification in field populations (Diatom Silicification)

Coverage: Oregon/California Coastal Upwelling Zone, between 34-44N and 120-124W


Description from NSF award abstract: Diatoms, unicellular, eukaryotic photoautotrophs, are among the most ecologically successful and functionally diverse organisms in the ocean. In addition to contributing one-fifth of total global primary productivity, diatoms are also the largest group of silicifying organisms in the ocean. Thus, diatoms form a critical link between the carbon and silicon (Si) cycles. The goal of this project is to understand the molecular regulation of silicification processes in natural diatom populations to better understand the processes controlling diatom productivity in the sea. Through culture studies and two research cruises, this research will couple classical measurements of silicon uptake and silica production with molecular and biochemical analyses of Silicification-Related Gene (SiRG) and protein expression. The proposed cruise track off the West Coast of the US will target gradients in Si and iron (Fe) concentrations with the following goals: 1) Characterize the expression pattern of SiRGs, 2) Correlate SiRG expression patterns to Si concentrations, silicon uptake kinetics, and silica production rates, 3) Develop a method to normalize uptake kinetics and silica production to SiRG expression levels as a more accurate measure of diatom activity and growth, 4) Characterize the diel periodicity of silica production and SiRG expression. It is estimated that diatoms process 240 Teramoles of biogenic silica each year and that each molecule of silicon is cycled through a diatom 39 times before being exported to the deep ocean. Decades of oceanographic and field research have provided detailed insight into the dynamics of silicon uptake and silica production in natural populations, but a molecular understanding of the factors that influence silicification processes is required for further understanding the regulation of silicon and carbon fluxes in the ocean. Characterizing the genetic potential for silicification will provide new information on the factors that regulate the distribution of diatoms and influence in situ rates of silicon uptake and silica production. This research is expected to provide significant information about the molecular regulation of silicification in natural populations and the physiological basis of Si limitation in the sea.


[ table of contents | back to top ]

Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

[ table of contents | back to top ]

This document is created by info v 4.1f 5 Oct 2018 from the content of the BCO-DMO metadata database.    2020-04-09  05:42:21