|Olson, Brady M.||Western Washington University (WWU)||Principal Investigator|
|Love, Brooke||Western Washington University (WWU)||Co-Principal Investigator|
|Strom, Suzanne||Western Washington University (WWU)||Co-Principal Investigator|
|Kendall, Kasey||Western Washington University (WWU)||Student|
|Copley, Nancy||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
These data are unprocessed Emiliania huxleyi cell volumes.
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.
The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.
Expt. 2: On day 8, after ~16 generations, E. huxleyi cells from the treatments were mounted live on a microscope slide and 200 cells from each treatment were imaged using RSImage software under 400X magnification on an Olympus CHA microscope.
Expt. 3: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were mounted live on a microscope slide and 195-204 cells from each treatment were imaged using RSImage software under 400X magnification on an Olympus CHA microscope.
Since E. huxleyi cells are rough spheres, the volume of the spherical was calculated using: V(µm3) = 4/3 πr3. The radius was calculated as: r(µm)=square root of (A/π). The area, A, was determined from the 2-D images using ImageJ software.
- added conventional header with dataset name, PI name, version date
- column names reformatted to comply with BCO-DMO standards
- added column 'expt' for experiment number
- experiments 2 and 3 data were concatenated into one data set
|treatment_cell_number||pCO2 level (ambient; moderate; high) and cell id number||unitless|
|cell_area_um||area of the cell||micrometers^2|
|cell_radius_um||radius of the cell||micrometers|
|cell_volume_um||volume of the cell||micrometers^3|
|Dataset-specific Instrument Name|
|Generic Instrument Name|| |
|Dataset-specific Description|| |
Olympus CHA microscope
|Generic Instrument Description|| |
Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
|Start Date|| |
|End Date|| |
This document is created by info v 4.1f 5 Oct 2018 from the content of the BCO-DMO metadata database. 2020-04-09 07:57:17