|Olson, Brady M.||Western Washington University (WWU)||Principal Investigator|
|Love, Brooke||Western Washington University (WWU)||Co-Principal Investigator|
|Strom, Suzanne||Western Washington University (WWU)||Co-Principal Investigator|
|Still, Kelly Ann||Western Washington University (WWU)||Student|
|Copley, Nancy||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO2 induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet
The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. On days 10 and 16, Rhodomonas cells from the treatments were mounted live on a microscope slide and 50 cells from each treatment replicate were imaged using RSImage software under 400X magnification on an Olympus CHA microscope. ImageJ software was used to measure Rhodomonas length and width. Rhodomonas cells are described as having a prolate spheroid shape. The volume was calculated using: Vprolate(µm3) =(4/3)πa2b. Where a=1/2 width and b=1/2 length of the Rhodomonas cell.
These data are unprocessed cell sizes as calculated above.
BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- combined day 10 and day 16 data
- added day column
|expt_day_treatment_rep_cell_number||sample identifier: individual cell measured: experiment day_pCO2 level_replicate_cell number||unitless|
|Dataset-specific Instrument Name|| |
Olympus CHA microscope
|Generic Instrument Name|| |
|Dataset-specific Description|| |
Used to measure Rhodomonas cells
|Generic Instrument Description|| |
Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
|Start Date|| |
|End Date|| |
This document is created by info v 4.1f 5 Oct 2018 from the content of the BCO-DMO metadata database. 2020-04-04 06:47:24