|Johnson, Zackary I.||Duke University||Principal Investigator|
|Rauch, Shannon||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Total bacterioplankton ("bacteria"), including Archaea and Prochlorococcus, determined by flow cytometry from the POWOW1 (TN277) cruise.
Bacterioplankton (i.e. ‘bacteria’) were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010). Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (<15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (> 670 nm) emissions were measured. Bacterioplankton were quantified by staining the samples with the nucleic acid stain SYBR Green –I (Molecular Probes Inc.) (Marie et al., 1997).
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. (2010) The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: 10.1357/002224010793721433
Marie, D., Partensky, F., Jacquet, S., and Vaulot, D. (1997) Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Applied and Environmental Microbiology 63: 186-193.
Cells counts were normalized to volume sampled to determined cells per mL.
BCO-DMO edits made:
- Parameter names have been changed to conform to BCO-DMO conventions.
- month_utc, day_utc, and year were added, based on the original date field.
- Original version of data (dated 22 March 2013) replaced with updated version 28 May 2013.
|cruise_id||Cruise identifier (POWOW1 = TN277 = R/V Thomas G. Thompson cruise 277).||text|
|CTD_cast||Number of CTD cast.||unitless|
|lat||Latitude in decimal degrees. Positive = North.||decimal degrees|
|lon||Longitude in decimal degrees. Positive = East.||decimal degrees|
|month_utc||2-digit month of year, UTC.||mm (01 to 12)|
|day_utc||2-digit day of month, UTC.||dd (01 to 31)|
|bot||Rosette position of the bottle.||unitless|
|bacteria||Bacteria (cells/mL) including Archaea and Prochlorococcus.||cells per milliliter|
|Dataset-specific Instrument Name|| |
|Generic Instrument Name|| |
|Dataset-specific Description|| |
A FACSCalibur flow cytometer (Becton Dickinson) was used to enumerate bacteria.
|Generic Instrument Description|| |
Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
R/V Thomas G. Thompson
|Start Date|| |
|End Date|| |
The POWOW#1 cruise was a trip of opportunity to sample along temperature gradients and test out new protocols. The primary goal of this cruise was to measure the abundance, diversity and activity of Prochlorococcus and associated bacterial and viral communities across temperature (and other environmental) gradients to understand how climate change may impact ocean ecology and biogeochemistry. There are many additional scientific and broader impact goals including characterizing oxidative stress and investigating nitrogen uptake/utilization molecular diversity. Cruise information and original data are available from the NSF R2R data catalog.
This document is created by info v 4.1f 5 Oct 2018 from the content of the BCO-DMO metadata database. 2020-01-23 07:09:08