|Krause, Jeffrey W||Dauphin Island Sea Lab (DISL)||Principal Investigator, Contact|
|Brzezinski, Mark A.||University of California-Santa Barbara (UCSB)||Co-Principal Investigator|
|York, Amber||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
This dataset contains silica uptake rates from Synechococcus cyanobacteria cultures grown in Sargasso Sea water with and without added orthophosphate. Experiments took place at the Krause Lab at Dauphin Island Sea Laboratory (30.2501,-88.0788) between August of 2013 to September of 2014.
These results are also presented in the following paper:
Brzezinski, M. A., Krause, J. W., Baines, S. B., Collier, J. L., Ohnemus, D. C. and Twining, B. S. (2017), Patterns and regulation of silicon accumulation in Synechococcus spp.. J. Phycol., 53: 746–761. doi:10.1111/jpy.12545
Monocultures of four Synechococcus clones (1333, 1334, 2515, 2370) were used to examine variation and controls on Si quotas and rates of Si accumulation. Cultures were procured from the National Center for Marine Algae and Microbiota (NCMA) at the Bigelow Laboratory for Ocean Sciences in Boothbay Harbor, Maine. Many of these clones are also available in other culture collections and have various strain names; here we will refer to each by their NCMA strain number (a.k.a. CCMP number).
Cultures were kept in surface Sargasso seawater (ambient concentrations ~1 μM Si(OH)4, ~0.1 uM PO4) with added orthophosphate (f/2 level, 36 μM) as well as the same seawater without added orthophosphate.
Each incubation was terminated by filtration and processed for measurement of 32Si activity following Krause et al. (2011). After aging for 120 days, secular equilibrium between 32Si and its daughter 32P was achieved and 32Si activity was determined using gas-flow proportional counting using GM 25-5 multicounters (Risø National Laboratory, Technical University of Denmark).
Full details of culturing and experimental methods are described in Brzezinski et al. (in review). (as of 05 Jan 2017)
BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values replaced with no data value 'nd'
* latitude and longitude of Dauphin Island Sea Lab added to dataset
* Si uptake rate limited to 5 decimal places
|clone_id||Synechococcus clone identifier (NCMA strain and CCMP number)||unitless|
|clone_lat||Latitude of the clone collection site||decimal degrees|
|clone_lon||Longitude of the clone collection site||decimal degrees|
|treatment||Shorthand code for additions to culture medium (e.g. N means added nitrate)||unitless|
|uptake_rate||Silica uptake rate||reciprocal hours (h-1)|
|treatment_descrip||Description of what additions were made to culture medium||untiless|
|Dataset-specific Instrument Name|| |
GM 25-5 multicounter
|Generic Instrument Name|| |
|Dataset-specific Description|| |
Manufactured by Riso Laboratories
|Generic Instrument Description|| |
A gas flow multicounter (GM multicounter) is used for counting low-level beta doses. GM multicounters can be used for gas proportional counting of 32Si to 32P. For more information about GM multicounter usage see Krause et. al. 2011 .
|Start Date|| |
|End Date|| |
Laboratory experiments conducted at Dauphin Island sea lab. Clone collection locations included in deployment coordinates.
This document is created by info v 4.1f 5 Oct 2018 from the content of the BCO-DMO metadata database. 2020-02-18 13:24:19