# Inhibition data for 3 peptidases at # Bogue Sound NC and Tennessee River at Knoxville # PI: Andrew D. Steen (University of Tennessee Knoxville) # 14 March 2016 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Ala A -113.66 Ala-pNA neutral_nonpolar 1.8 89.09 0.1 -0.043 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.00289406522495565 0.00056516458212828 0.76623263654501 0.000906354631022907 FALSE 3.73669352062653 0.729716391294778 3.00697712933176 4.46640991192131 TN_River Leu-AMC 0.00256896042294767 0.000609844142853933 0.689260597646688 0.00294585670118042 FALSE 3.98020658848213 0.944859116416176 3.03534747206595 4.9250657048983 TN_River Arg-AMC 0.00089972305823684 0.000326839724752409 0.486451477308213 0.0249509030802908 FALSE 11.5144997450379 4.18283814433396 7.3316616007039 15.6973378893718 Bogue_Sound Pro-AMC 0.0205498374527379 0.0155394481426008 0.179388029669718 0.222579303242916 FALSE 5.84157482735053 4.41730253630992 1.42427229104061 10.2588773636605 Bogue_Sound Leu-AMC -0.00257532068071111 0.00095851110583333 0.546102904090211 0.0362069855372132 FALSE 8.78156434958888 0.484394426578918 8.29716992300996 9.26595877616779 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Glu E -224.67 Glu-pNA acidic_polar -3.5 147.13 0.058 0.065 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.00992475770001952 0.00126390450758329 0.885158295304774 4.99173657866368e-05 FALSE 1.08962204430855 0.138761897770158 0.950860146538396 1.22838394207871 Bogue_Sound Leu-AMC 0.0134632831395076 0.00119494173520326 0.940715676386693 3.46086930416594e-06 FALSE 0.248950865100493 0.0220957827032979 0.226855082397195 0.271046647803791 Bogue_Sound Pro-AMC 0.056376167015518 0.0229821954952647 0.429280288081794 0.0397480458845094 FALSE 2.12932910350248 0.868038043753145 1.26129105974933 2.99736714725562 TN_River Leu-AMC 0.00915507084738567 0.00153804731578444 0.81579998776226 0.000341060062594781 FALSE 1.11686663832711 0.187633035702693 0.929233602624415 1.3044996740298 TN_River Arg-AMC 0.00415621335559696 0.000551828154911985 0.876403509891098 6.7231195431475e-05 FALSE 2.49262009389429 0.330949792425337 2.16167030146895 2.82356988631963 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Phe F -303.64 Phe-pNA neutral_nonpolar 2.8 165.19 0.122 0.134 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.0249890925920289 0.00189292331182594 0.956110257639476 1.03295316393143e-06 FALSE 0.432758201784882 0.0327814259571705 0.399976775827711 0.465539627742052 Bogue_Sound Leu-AMC 0.0209363805566608 0.00220318219344949 0.918618998824504 1.24051276802793e-05 FALSE 0.160089561593633 0.0168465829375658 0.143242978656067 0.176936144531199 Bogue_Sound Pro-AMC 0.0988997097502494 0.0296487257610683 0.581743018414974 0.0102965241457504 FALSE 1.21378933743288 0.363876772622838 0.849912564810038 1.57766611005571 TN_River Arg-AMC 0.00655845852323201 0.000322105045388282 0.985733991267264 9.12209588405711e-07 FALSE 1.57961827279613 0.0775796650467216 1.5020386077494 1.65719793784285 TN_River Leu-AMC 0.00841010683630106 0.00352373044014198 0.41590333837272 0.0440819082152346 FALSE 1.21579825321973 0.509404386570916 0.706393866648813 1.72520263979065 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Gly G -80.49 Gly-pNA neutral_nonpolar -0.4 75.07 -0.026 -0.099 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.0102647894145204 0.000789358782473322 0.954828534700769 1.15962254381965e-06 FALSE 1.05352719258563 0.0810158794748914 0.972511313110735 1.13454307206052 Bogue_Sound Leu-AMC -0.00190144466540051 0.002154802410425 0.0887000448235717 0.403283646149579 FALSE 8.78156434958888 1.99758233600844 6.78398201358043 10.7791466855973 Bogue_Sound Pro-AMC 0.0235364831504617 0.0115943239597217 0.370554650700081 0.0819127189807264 FALSE 5.10031224302532 2.51246849681578 2.58784374620953 7.6127807398411 TN_River Arg-AMC 0.000533489976343308 0.00142146591459469 0.0197255566554322 0.718543836291828 FALSE 19.4190357533654 51.7413609284785 0.01 71.1603966818439 TN_River Leu-AMC 0.00857076331281858 0.00137809693176923 0.828618107504461 0.000254042187061861 FALSE 1.19300846701407 0.191824374091382 1.00118409292269 1.38483284110545 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Ile I -213.93 Ile-pNA neutral_nonpolar 4.5 131.17 0.126 0.139 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.0113078073135543 0.00112508998262562 0.926615042023282 8.17429291007344e-06 FALSE 0.956351171760739 0.0951538254397507 0.861197346320988 1.05150499720049 Bogue_Sound Leu-AMC 0.00195113323905484 0.0013458219355799 0.208064684843591 0.185166433296526 FALSE 1.71782014553599 1.18489090697019 0.532929238565795 2.90271105250618 Bogue_Sound Pro-AMC 0.0307959079865849 0.0240079516479674 0.170590495940468 0.235498272728146 FALSE 3.89803129761108 3.03883707394102 0.859194223670059 6.9368683715521 TN_River Arg-AMC 0.00385885767320472 0.00115243639907581 0.58359386644559 0.0101037606495957 FALSE 2.68469630186414 0.801776587982577 1.88291971388156 3.48647288984672 TN_River Leu-AMC 0.0094231888884382 0.00155296380175695 0.821504657231657 0.000299920963264299 FALSE 1.08508842622392 0.178825137390454 0.906263288833466 1.26391356361437 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Lys K -258.56 Lys-pNA basic_polar -3.9 146.19 nd nd ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.00677038901387648 0.00190656800625899 0.611843046119491 0.00749753931938233 FALSE 1.59728410763365 0.44980144719581 1.14748266043784 2.04708555482946 Bogue_Sound Leu-AMC 0.00261524282100014 0.00132507952095355 0.327465085314142 0.0838687934167093 FALSE 1.28160030027021 0.64935550087335 0.632244799396861 1.93095580114356 Bogue_Sound Pro-AMC 0.0253299414403478 0.0154718629929196 0.250957258961765 0.140232405066091 FALSE 4.73919031564924 2.8947600780592 1.84443023759004 7.63395039370844 TN_River Arg-AMC 0.00857641327259424 0.000651374530279463 0.955888932712963 1.05404393065652e-06 FALSE 1.20794796092412 0.0917430761135629 1.11620488481056 1.29969103703768 TN_River Leu-AMC 0.0194468450993206 0.00314971575043515 0.826540351467987 0.000266847614110437 FALSE 0.525791877743878 0.0851600838245285 0.44063179391935 0.610951961568407 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Leu L -205.03 Leu-pNA neutral_nonpolar 3.8 131.17 0.122 0.169 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.023785660728531 0.00335587254250407 0.86262914811759 0.000103232762016527 FALSE 0.454653536758415 0.0641461819275753 0.39050735483084 0.518799718685991 Bogue_Sound Leu-AMC 0.0033516959846733 0.00213204848829993 0.236010982093188 0.154581658121584 TRUE 1 0.636110344747674 0.363889655252326 1.63611034474767 Bogue_Sound Pro-AMC 0.0440279589969691 0.0255527918913853 0.27065834503175 0.123180065081572 FALSE 2.72652686849106 1.58241206824097 1.14411480025009 4.30893893673203 TN_River Arg-AMC 0.00415860822830203 0.00075829667113711 0.789892995318282 0.00058458678044252 FALSE 2.49118463580368 0.454252219206797 2.03693241659688 2.94543685501048 TN_River Leu-AMC 0.0102249932009661 0.00200271776890265 0.765166777140834 0.000923490647058168 TRUE 1 0.195864948713454 0.804135051286546 1.19586494871345 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Met M -113.22 Met-pNA neutral_nonpolar 1.9 149.21 0.071 0.134 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.036472478587003 0.00227711400316162 0.96975922623511 2.31500415509814e-07 FALSE 0.296503972126972 0.0185118580661521 0.27799211406082 0.315015830193125 Bogue_Sound Leu-AMC 0.0230045707859732 0.0023339041593378 0.923921356649569 9.45306840270015e-06 FALSE 0.145696958046135 0.0147815292686999 0.130915428777436 0.160478487314835 Bogue_Sound Pro-AMC 0.142021884993831 0.0264879720313071 0.782303230698214 0.000676252800465736 FALSE 0.845245880064704 0.157643656340035 0.68760222372467 1.00288953640474 TN_River Arg-AMC 0.00827017276784964 0.00125844669737448 0.843712283699173 0.000174460142544856 FALSE 1.25267769071848 0.190616102832548 1.06206158788594 1.44329379355103 TN_River Leu-AMC 0.0177774661675388 0.00182354916830363 0.92235997449712 1.02601731513168e-05 FALSE 0.575165949106782 0.0589984747120713 0.51616747439471 0.634164423818853 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Pro P -192.83 Pro-pNA neutral_nonpolar -1.6 115.13 nd nd ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Leu-AMC -0.00173407338472435 0.00124977505827219 0.19396924119691 0.202711735849762 FALSE 8.78156434958888 1.39303339297544 7.38853095661344 10.1745977425643 Bogue_Sound Arg-AMC 0.00503352019198059 0.000725046615045531 0.857641331364616 0.000119332538183232 FALSE 2.14844370577704 0.309469670742811 1.83897403503423 2.45791337651985 Bogue_Sound Pro-AMC 0.120043413170059 0.0225392459076479 0.780014223235296 0.000705941897301524 TRUE 1 0.187759122407806 0.812240877592194 1.18775912240781 TN_River Arg-AMC 0.00113643432146432 0.000318270696716257 0.679992578677723 0.011774907643171 FALSE 9.11611056530206 2.55306514961868 6.56304541568338 11.6691757149207 TN_River Leu-AMC 0.00476483946072827 0.00174591202967437 0.48214060208061 0.025880173603001 FALSE 2.14592606639539 0.786301021260192 1.3596250451352 2.93222708765558 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Arg R -409.46 Arg-pNA basic_polar -4.5 174.2 -0.028 -0.115 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Arg-AMC 0.0108142347743623 0.00111645231583001 0.921432608469315 1.07635104912095e-05 TRUE 1 0.103239141661398 0.896760858338602 1.1032391416614 TN_River Leu-AMC 0.0211557429190322 0.00343329490359091 0.825971391341677 0.00027043827443483 FALSE 0.483319977941663 0.0784363859695972 0.404883591972066 0.56175636391126 TN_River Arg-AMC 0.0103598609246728 0.00127309488894668 0.892211705042653 3.86176331285027e-05 TRUE 1 0.122887256711595 0.877112743288405 1.12288725671159 Bogue_Sound Leu-AMC 0.00757532343608537 0.00156447456319442 0.745594133616584 0.00128471264698719 FALSE 0.442449225165404 0.0913757101088292 0.351073515056575 0.533824935274233 Bogue_Sound Pro-AMC 0.0155454336095046 0.0155262146626524 0.125270860896917 0.350058310346989 FALSE 7.72210130547042 7.71255440840052 0.00954689706989509 15.4346557138709 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Val V -178 Val-pNA neutral_nonpolar 4.2 117.15 0.054 -0.044 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- TN_River Arg-AMC 0.00145939183115609 0.000381269305802963 0.646821477992217 0.00503419721695866 FALSE 7.09875216751486 1.85456452009302 5.24418764742184 8.95331668760788 Bogue_Sound Pro-AMC 0.0475731015670468 0.0193402600724356 0.430629223767767 0.03933124651236 FALSE 2.52334637044584 1.02583547109032 1.49751089935552 3.54918184153616 Bogue_Sound Leu-AMC -0.000253709297766955 0.00125602092608788 0.0057950461525848 0.845665981206945 FALSE 8.78156434958888 65.4016533926753 0.01 74.1832177422641 Bogue_Sound Arg-AMC 0.00373113597758224 0.000754137069120692 0.753681827027265 0.00112433048013635 FALSE 2.89837594752307 0.585819641902017 2.31255630562106 3.48419558942509 TN_River Leu-AMC 0.00127501266911058 0.0006501083097267 0.324690932219743 0.0854951708651589 FALSE 8.01952282411346 4.08902480289545 3.93049802121802 12.1085476270089 ========================= amino_acid AA_abbrev delta_Gr pNA_subs polarity hydrophobicity MW Dauwe_score1 Dauwe_score2 ------------------------- Tyr Y -334.2 Tyr-pNA neutral polar -1.3 181.19 0.188 0.178 ========================= location substrate inhib_slope inhib_slope_SE rsq pval is_homologous rel_Ki rel_Ki_SE low_err high_err ------------------------- Bogue_Sound Pro-AMC 0.0810965630199684 0.025843754712359 0.551739547638354 0.0138478975843062 FALSE 1.4802527838387 0.471725168530194 1.00852761530851 1.9519779523689 TN_River Arg-AMC 0.00529363252553339 0.00044413809651492 0.946687958457449 2.25754927962203e-06 FALSE 1.95704195081598 0.164196680189408 1.79284527062657 2.12123863100539 Bogue_Sound Leu-AMC 0.0220500459002534 0.00306224558772631 0.866329884775689 9.23952080656539e-05 FALSE 0.152004036628095 0.0211098739923995 0.130894162635696 0.173113910620495 Bogue_Sound Arg-AMC 0.0185973062886412 0.00177879502663259 0.931802920032291 6.08311521185688e-06 FALSE 0.581494685656032 0.055618799776933 0.525875885879099 0.637113485432965 TN_River Leu-AMC 0.0173638808897526 0.00184821908692538 0.916895746828351 1.34996980546865e-05 FALSE 0.58886566118986 0.0626791188880091 0.526186542301851 0.651544780077869