# CO2 x temperature effects on Menidia menidia offspring # PI: Hannes Baumann (UConn) # Co-PIs: Christopher Murray (UConn) & Janet Nye (SUNY Stony Brook) # Version: 05 April 2018 ========================= experiment species adult_collection_site latitude longitude ------------------------- 1 Menidia_menidia Poquot_Beach_NY 40.947376 -73.10258 ========================= tank replicate temperature pH pCO2 fertilization_date hatch_sample_date hatch_sample_age final_sample_date final_sample_age rel_embryo_survival rel_larval_survival hatch_length final_length growth_rate ------------------------- A 1 24.0 7.14 6195 2014/05/05 2014/05/12 7 2014/05/21 16 0.49 0.85 nd nd nd A 2 24.0 7.14 6195 2014/05/05 2014/05/12 7 2014/05/21 16 0.60 0.12 nd nd nd A 3 24.0 7.14 6195 2014/05/05 2014/05/12 7 2014/05/21 16 0.58 0.35 nd nd nd A 4 24.0 7.14 6195 2014/05/05 2014/05/12 7 2014/05/21 16 0.48 0.32 nd nd nd A 5 24.0 7.14 6195 2014/05/05 2014/05/12 7 2014/05/21 16 0.66 0.20 nd nd nd B 6 24.0 7.47 6195 2014/05/05 2014/05/12 7 2014/05/21 16 0.61 0.75 nd nd nd B 7 24.0 7.47 2881 2014/05/05 2014/05/12 7 2014/05/21 16 0.69 0.41 nd nd nd B 8 24.0 7.47 2881 2014/05/05 2014/05/12 7 2014/05/21 16 0.66 0.50 nd nd nd B 9 24.0 7.47 2881 2014/05/05 2014/05/12 7 2014/05/21 16 0.65 0.45 nd nd nd B 10 24.0 7.47 2881 2014/05/05 2014/05/12 7 2014/05/21 16 0.40 0.57 nd nd nd C 11 24.0 8.20 474 2014/05/05 2014/05/12 7 2014/05/21 16 0.66 0.36 nd nd nd C 12 24.0 8.20 474 2014/05/05 2014/05/12 7 2014/05/21 16 0.69 0.53 nd nd nd C 13 24.0 8.20 474 2014/05/05 2014/05/12 7 2014/05/21 16 0.68 0.38 nd nd nd C 14 24.0 8.20 474 2014/05/05 2014/05/12 7 2014/05/21 16 0.61 0.37 nd nd nd C 15 24.0 8.20 474 2014/05/05 2014/05/12 7 2014/05/21 16 0.61 0.57 nd nd nd D 16 17.5 7.14 5753 2014/05/05 2014/05/19 14 2014/05/31 26 0.64 0.11 nd nd nd D 17 17.5 7.14 5753 2014/05/05 2014/05/19 14 2014/05/31 26 0.49 0.72 nd nd nd D 18 17.5 7.14 5753 2014/05/05 2014/05/19 14 2014/05/31 26 0.58 0.48 nd nd nd D 19 17.5 7.14 5753 2014/05/05 2014/05/19 14 2014/05/31 26 0.50 0.18 nd nd nd D 20 17.5 7.14 5753 2014/05/05 2014/05/19 14 2014/05/31 26 0.60 0.68 nd nd nd E 21 17.5 7.49 2564 2014/05/05 2014/05/19 14 2014/05/31 26 0.72 0.73 nd nd nd E 22 17.5 7.49 2564 2014/05/05 2014/05/19 14 2014/05/31 26 0.79 0.39 nd nd nd E 23 17.5 7.49 2564 2014/05/05 2014/05/19 14 2014/05/31 26 0.73 0.68 nd nd nd E 24 17.5 7.49 2564 2014/05/05 2014/05/19 14 2014/05/31 26 0.76 0.20 nd nd nd E 25 17.5 7.49 2564 2014/05/05 2014/05/19 14 2014/05/31 26 0.72 0.50 nd nd nd F 26 17.5 8.24 433 2014/05/05 2014/05/19 14 2014/05/31 26 0.72 0.42 nd nd nd F 27 17.5 8.24 433 2014/05/05 2014/05/19 14 2014/05/31 26 0.66 0.30 nd nd nd F 28 17.5 8.24 433 2014/05/05 2014/05/19 14 2014/05/31 26 0.61 0.27 nd nd nd F 29 17.5 8.24 433 2014/05/05 2014/05/19 14 2014/05/31 26 0.70 0.25 nd nd nd F 30 17.5 8.24 433 2014/05/05 2014/05/19 14 2014/05/31 26 0.69 0.44 nd nd nd ========================= experiment species adult_collection_site latitude longitude ------------------------- 2 Menidia_menidia Mumford_Cove_CT 41.321526 -72.015247 ========================= tank replicate temperature pH pCO2 fertilization_date hatch_sample_date hatch_sample_age final_sample_date final_sample_age rel_embryo_survival rel_larval_survival hatch_length final_length growth_rate ------------------------- E 1 16.9 8.17 368 2016/04/22 2016/05/07 15 2016/05/22 30 0.95 0.13 5.33 8.79 0.23 E 2 16.9 8.17 368 2016/04/22 2016/05/07 15 2016/05/22 30 0.89 0.29 5.38 7.55 0.14 E 3 16.9 8.17 368 2016/04/22 2016/05/07 15 2016/05/22 30 0.89 0.32 5.29 7.65 0.16 E 4 16.9 8.17 368 2016/04/22 2016/05/07 15 2016/05/22 30 0.94 0.15 5.26 8.34 0.21 E 5 16.9 8.17 368 2016/04/22 2016/05/07 15 2016/05/22 30 0.39 0.17 5.12 7.90 0.19 F 7 16.9 7.49 2037 2016/04/22 2016/05/07 15 2016/05/22 30 0.69 0.10 5.27 8.88 0.24 F 8 16.9 7.49 2037 2016/04/22 2016/05/07 15 2016/05/22 30 0.90 0.23 5.31 7.39 0.14 F 9 16.9 7.49 2037 2016/04/22 2016/05/07 15 2016/05/22 30 0.90 0.05 5.22 7.39 0.14 F 10 16.9 7.49 2037 2016/04/22 2016/05/07 15 2016/05/22 30 0.85 0.08 5.32 7.37 0.14 F 11 16.9 7.49 2037 2016/04/22 2016/05/07 15 2016/05/22 30 0.99 0.08 5.34 7.67 0.16 G 13 23.5 8.13 427 2016/04/22 2016/04/29 7 2016/05/08 16 0.78 0.09 5.13 8.10 0.33 G 14 23.5 8.13 427 2016/04/22 2016/04/29 7 2016/05/08 16 0.92 0.04 5.42 9.63 0.47 G 15 23.5 8.13 427 2016/04/22 2016/04/29 7 2016/05/08 16 0.86 0.63 5.37 9.34 0.44 G 16 23.5 8.13 427 2016/04/22 2016/04/29 7 2016/05/08 16 0.86 0.71 5.18 9.00 0.42 G 17 23.5 8.13 427 2016/04/22 2016/04/29 7 2016/05/08 16 0.97 0.11 5.41 9.10 0.41 H 19 23.4 7.49 2190 2016/04/22 2016/04/29 7 2016/05/08 16 0.81 0.31 5.41 9.69 0.48 H 20 23.4 7.49 2190 2016/04/22 2016/04/29 7 2016/05/08 16 0.81 0.23 5.40 8.81 0.38 H 21 23.4 7.49 2190 2016/04/22 2016/04/29 7 2016/05/08 16 0.77 0.34 5.37 8.71 0.37 H 22 23.4 7.49 2190 2016/04/22 2016/04/29 7 2016/05/08 16 0.71 0.26 5.29 8.97 0.41 H 23 23.4 7.49 2190 2016/04/22 2016/04/29 7 2016/05/08 16 0.68 0.17 5.22 8.46 0.36 ========================= experiment species adult_collection_site latitude longitude ------------------------- 3 Menidia_menidia Mumford_Cove_CT 41.321526 -72.015247 ========================= tank replicate temperature pH pCO2 fertilization_date hatch_sample_date hatch_sample_age final_sample_date final_sample_age rel_embryo_survival rel_larval_survival hatch_length final_length growth_rate ------------------------- 1 55 19.7 8.20 345 2016/05/19 2016/05/30 11 2016/06/13 25 0.90 0.74 5.56 10.48 0.35 1 56 19.7 8.20 345 2016/05/19 2016/05/30 11 2016/06/13 25 0.99 0.69 5.74 10.57 0.35 1 57 19.7 8.20 345 2016/05/19 2016/05/30 11 2016/06/13 25 1.00 0.89 5.45 9.92 0.32 1 58 19.7 8.20 345 2016/05/19 2016/05/30 11 2016/06/13 25 1.00 0.86 5.50 10.15 0.33 1 59 19.7 8.20 345 2016/05/19 2016/05/30 11 2016/06/13 25 0.91 0.91 5.51 9.76 0.30 2 60 19.6 7.51 1964 2016/05/19 2016/05/30 11 2016/06/13 25 0.87 0.66 5.53 10.43 0.35 2 61 19.6 7.51 1964 2016/05/19 2016/05/30 11 2016/06/13 25 0.93 0.92 5.62 10.26 0.33 2 62 19.6 7.51 1964 2016/05/19 2016/05/30 11 2016/06/13 25 1.00 0.78 5.71 10.07 0.31 2 63 19.6 7.51 1964 2016/05/19 2016/05/30 11 2016/06/13 25 1.00 0.90 5.72 9.88 0.30 2 64 19.6 7.51 1964 2016/05/19 2016/05/30 11 2016/06/13 25 0.96 0.60 5.55 10.01 0.32 3 65 19.7 7.21 4066 2016/05/19 2016/05/30 11 2016/06/13 25 0.83 1.00 5.11 10.40 0.38 3 66 19.7 7.21 4066 2016/05/19 2016/05/30 11 2016/06/13 25 0.92 0.78 5.32 10.37 0.36 3 67 19.7 7.21 4066 2016/05/19 2016/05/30 11 2016/06/13 25 0.98 0.92 5.48 9.49 0.29 3 68 19.7 7.21 4066 2016/05/19 2016/05/30 11 2016/06/13 25 1.00 0.48 5.39 10.20 0.34 3 69 19.7 7.21 4066 2016/05/19 2016/05/30 11 2016/06/13 25 0.98 0.57 5.53 9.92 0.31 4 70 23.6 7.20 4339 2016/05/19 2016/05/26 7 2016/06/04 16 0.98 0.83 5.29 7.96 0.30 4 71 23.6 7.20 4339 2016/05/19 2016/05/26 7 2016/06/04 16 0.98 0.59 5.09 8.37 0.36 4 72 23.6 7.20 4339 2016/05/19 2016/05/26 7 2016/06/04 16 0.98 0.75 5.13 8.71 0.40 4 73 23.6 7.20 4339 2016/05/19 2016/05/26 7 2016/06/04 16 0.86 0.49 5.36 8.81 0.38 4 74 23.6 7.20 4339 2016/05/19 2016/05/26 7 2016/06/04 16 0.79 0.77 5.23 9.04 0.42 5 75 23.7 7.49 2157 2016/05/19 2016/05/26 7 2016/06/04 16 0.88 0.73 5.26 8.80 0.39 5 76 23.7 7.49 2157 2016/05/19 2016/05/26 7 2016/06/04 16 0.88 0.69 5.29 8.65 0.37 5 77 23.7 7.49 2157 2016/05/19 2016/05/26 7 2016/06/04 16 0.99 0.70 5.39 8.34 0.33 5 78 23.7 7.49 2157 2016/05/19 2016/05/26 7 2016/06/04 16 1.00 0.91 5.32 8.23 0.32 5 79 23.7 7.49 2157 2016/05/19 2016/05/26 7 2016/06/04 16 0.98 0.69 5.34 8.41 0.34 6 80 23.7 8.22 331 2016/05/19 2016/05/26 7 2016/06/04 16 0.87 0.65 5.49 9.15 0.41 6 81 23.7 8.22 331 2016/05/19 2016/05/26 7 2016/06/04 16 0.97 0.66 5.53 8.73 0.36 6 82 23.7 8.22 331 2016/05/19 2016/05/26 7 2016/06/04 16 0.98 0.83 5.39 8.90 0.39 6 83 23.7 8.22 331 2016/05/19 2016/05/26 7 2016/06/04 16 0.95 0.78 5.65 8.94 0.37 6 84 23.7 8.22 331 2016/05/19 2016/05/26 7 2016/06/04 16 0.99 0.71 5.49 8.69 0.36 7 85 17.4 8.22 322 2016/05/19 2016/06/03 15 2016/06/24 36 0.94 0.38 5.42 9.89 0.21 7 86 17.4 8.22 322 2016/05/19 2016/06/03 15 2016/06/24 36 0.95 0.25 5.36 10.03 0.22 7 87 17.4 8.22 322 2016/05/19 2016/06/03 15 2016/06/24 36 1.00 0.39 5.30 10.35 0.24 7 88 17.4 8.22 322 2016/05/19 2016/06/03 15 2016/06/24 36 0.87 0.38 5.40 9.82 0.21 7 89 17.4 8.22 322 2016/05/19 2016/06/03 15 2016/06/24 36 0.91 0.22 5.39 9.30 0.19 8 90 17.6 7.51 1952 2016/05/19 2016/06/03 15 2016/06/24 36 0.88 0.28 5.42 9.01 0.17 8 91 17.6 7.51 1952 2016/05/19 2016/06/03 15 2016/06/24 36 0.93 0.78 5.43 9.84 0.21 8 92 17.6 7.51 1952 2016/05/19 2016/06/03 15 2016/06/24 36 1.00 0.63 5.56 10.02 0.21 8 93 17.6 7.51 1952 2016/05/19 2016/06/03 15 2016/06/24 36 0.95 0.39 5.44 9.41 0.19 8 94 17.6 7.51 1952 2016/05/19 2016/06/03 15 2016/06/24 36 1.00 0.69 5.23 9.65 0.21 9 95 17.4 7.20 4056 2016/05/19 2016/06/03 15 2016/06/24 36 0.87 0.62 5.30 9.11 0.18 9 96 17.4 7.20 4056 2016/05/19 2016/06/03 15 2016/06/24 36 0.87 0.64 5.58 9.75 0.20 9 97 17.4 7.20 4056 2016/05/19 2016/06/03 15 2016/06/24 36 0.82 0.74 5.37 9.47 0.20 9 98 17.4 7.20 4056 2016/05/19 2016/06/03 15 2016/06/24 36 0.91 0.60 5.36 9.46 0.20 9 99 17.4 7.20 4056 2016/05/19 2016/06/03 15 2016/06/24 36 0.98 0.35 5.47 9.21 0.18 ========================= experiment species adult_collection_site latitude longitude ------------------------- 4 Menidia_menidia Mumford_Cove_CT 41.321526 -72.015247 ========================= tank replicate temperature pH pCO2 fertilization_date hatch_sample_date hatch_sample_age final_sample_date final_sample_age rel_embryo_survival rel_larval_survival hatch_length final_length growth_rate ------------------------- B 1 23.6 8.19 368 2017/04/28 2017/05/04 6 2017/05/14 16 0.69 0.43 4.97 8.08 0.31 B 2 23.6 8.19 368 2017/04/28 2017/05/04 6 2017/05/14 16 0.52 0.33 4.92 8.62 0.37 B 3 23.6 8.19 368 2017/04/28 2017/05/04 6 2017/05/14 16 0.64 0.23 5.06 8.04 0.30 A 4 23.7 7.51 2155 2017/04/28 2017/05/04 6 2017/05/14 16 0.43 0.24 4.93 8.31 0.34 A 5 23.7 7.51 2155 2017/04/28 2017/05/04 6 2017/05/14 16 0.52 0.12 4.92 8.30 0.34 A 6 23.7 7.51 2155 2017/04/28 2017/05/04 6 2017/05/14 16 0.57 0.72 5.09 8.22 0.31 D 7 28.1 8.22 356 2017/04/28 2017/05/03 5 2017/05/12 14 0.50 0.08 4.72 9.80 0.56 D 8 28.1 8.22 356 2017/04/28 2017/05/03 5 2017/05/12 14 0.48 0.71 4.77 8.16 0.38 D 9 28.1 8.22 356 2017/04/28 2017/05/03 5 2017/05/12 14 0.40 0.13 4.79 9.17 0.49 C 10 27.9 7.52 2217 2017/04/28 2017/05/03 5 2017/05/12 14 0.52 0.14 4.70 8.64 0.44 C 11 27.9 7.52 2217 2017/04/28 2017/05/03 5 2017/05/12 14 0.46 0.36 4.64 9.32 0.52 C 12 27.9 7.52 2217 2017/04/28 2017/05/03 5 2017/05/12 14 0.48 0.68 4.53 7.82 0.37 ========================= experiment species adult_collection_site latitude longitude ------------------------- 5 Menidia_menidia Mumford_Cove_CT 41.321526 -72.015247 ========================= tank replicate temperature pH pCO2 fertilization_date hatch_sample_date hatch_sample_age final_sample_date final_sample_age rel_embryo_survival rel_larval_survival hatch_length final_length growth_rate ------------------------- A 1 24.3 7.21 4432 2017/05/26 2017/06/01 6 2017/06/11 16 0.85 0.31 4.85 7.61 0.28 A 2 24.3 7.21 4432 2017/05/26 2017/06/01 6 2017/06/11 16 0.55 0.76 4.74 8.64 0.39 A 3 24.3 7.21 4432 2017/05/26 2017/06/01 6 2017/06/11 16 0.54 0.18 4.76 8.55 0.38 A 4 24.3 7.21 4432 2017/05/26 2017/06/01 6 2017/06/11 16 0.53 0.09 4.92 7.96 0.30 A 5 24.3 7.21 4432 2017/05/26 2017/06/01 6 2017/06/11 16 0.60 0.10 4.91 7.98 0.31 A 6 24.1 7.50 2265 2017/05/26 2017/06/01 6 2017/06/11 16 0.66 0.36 4.92 7.94 0.30 A 7 24.1 7.50 2265 2017/05/26 2017/06/01 6 2017/06/11 16 0.98 0.36 4.88 8.16 0.33 A 8 24.1 7.50 2265 2017/05/26 2017/06/01 6 2017/06/11 16 0.92 0.69 4.65 8.83 0.42 A 9 24.1 7.50 2265 2017/05/26 2017/06/01 6 2017/06/11 16 0.80 0.29 5.05 8.72 0.37 B 10 24.1 7.50 2265 2017/05/26 2017/06/01 6 2017/06/11 16 0.79 0.16 5.03 9.04 0.40 B 11 24.2 8.19 389 2017/05/26 2017/06/01 6 2017/06/11 16 0.87 0.52 4.70 8.73 0.40 B 12 24.2 8.19 389 2017/05/26 2017/06/01 6 2017/06/11 16 0.87 0.21 4.85 7.65 0.28 B 13 24.2 8.19 389 2017/05/26 2017/06/01 6 2017/06/11 16 0.70 0.84 4.73 8.66 0.39 B 14 24.2 8.19 389 2017/05/26 2017/06/01 6 2017/06/11 16 0.58 0.20 4.77 7.49 0.27 B 15 24.2 8.19 389 2017/05/26 2017/06/01 6 2017/06/11 16 0.68 0.26 4.85 7.88 0.30 C 16 28.2 7.20 4720 2017/05/26 2017/05/31 5 2017/06/09 14 0.79 0.11 4.75 8.23 0.39 C 17 28.2 7.20 4720 2017/05/26 2017/05/31 5 2017/06/09 14 0.84 0.05 4.65 8.55 0.43 C 18 28.2 7.20 4720 2017/05/26 2017/05/31 5 2017/06/09 14 0.81 0.03 4.65 6.95 0.26 C 19 28.2 7.20 4720 2017/05/26 2017/05/31 5 2017/06/09 14 0.57 0.09 4.77 8.10 0.37 C 20 28.2 7.20 4720 2017/05/26 2017/05/31 5 2017/06/09 14 0.60 0.18 4.70 8.93 0.47 C 21 28.1 7.48 2439 2017/05/26 2017/05/31 5 2017/06/09 14 0.70 0.10 4.87 8.89 0.45 C 22 28.1 7.48 2439 2017/05/26 2017/05/31 5 2017/06/09 14 0.55 0.29 4.63 8.67 0.45 C 23 28.1 7.48 2439 2017/05/26 2017/05/31 5 2017/06/09 14 0.80 0.17 4.68 8.31 0.40 D 24 28.1 7.48 2439 2017/05/26 2017/05/31 5 2017/06/09 14 0.62 0.10 4.62 8.28 0.41 D 25 28.1 7.48 2439 2017/05/26 2017/05/31 5 2017/06/09 14 0.70 0.07 4.67 8.16 0.39 D 26 28.2 8.23 350 2017/05/26 2017/05/31 5 2017/06/09 14 0.83 0.15 4.42 7.85 0.38 D 27 28.2 8.23 350 2017/05/26 2017/05/31 5 2017/06/09 14 0.60 0.16 4.65 8.24 0.40 D 28 28.2 8.23 350 2017/05/26 2017/05/31 5 2017/06/09 14 0.89 0.23 4.61 8.58 0.44 D 29 28.2 8.23 350 2017/05/26 2017/05/31 5 2017/06/09 14 0.91 0.09 4.48 7.61 0.35 D 30 28.2 8.23 350 2017/05/26 2017/05/31 5 2017/06/09 14 0.76 0.06 4.52 8.42 0.43