# TriCoLim field enzyme-labeled fluorescence (ELF) analysis of Trichodesmium colonies # from RV/Atlantis cruise AT39-05, Feb-Mar 2018 # PI's: E. Webb, D. Hutchins (USC) # version date: 2020-05-06 # ========================= station lat lon date_local time_local slide_label colony_description Tricho_filament_thickness Tricho_filament_fluor Tricho_filament_ELF_glow ELF_reaction_results_plus_minus backgrnd_speckling backgrnd_speckling_filamaments backgrnd_speckling_colonies ------------------------- 2 12.50 -22.00 2018-02-13 13:20 slide Label (nd?) puff normal mostly orange, few green some lightly speckled/some moderately speckled +/- little little n/a 2 12.50 -22.00 2018-02-13 13:20 ELF puff #2 ASA 2/13/18 puff normal some orange, some green majority lightly speckled, some moderately speckled +/- little little n/a 2 12.50 -22.00 2018-02-13 13:20 ELF puff #3 ASA 2/13/18 puff normal some orange, some green none speckled - none none n/a 2 12.50 -22.00 2018-02-13 13:20 ELF puff #4 ASA 2/13/18 puff some normal, some thick some orange, some green none speckled - little little n/a 3 9.50 -22.00 2018-02-15 6:00 2/15/18 Puff #1 puff normal orange some lightly speckled +/- some some n/a 3 9.50 -22.00 2018-02-15 6:00 puff #2 puff normal mostly orange, few green few lightly speckled +/- some little n/a 3 9.50 -22.00 2018-02-15 6:00 2/15/18 Tuft #1 tuft normal mostly orange, some green few lightly speckled +/- some little n/a 3 9.50 -22.00 2018-02-15 6:00 2/15/18 Tuft #2 tuft some normal, some thick mostly orange, few green some lightly speckled +/- some some n/a 4 6.50 -22.00 2018-02-16 13:30 2/16/18 Puffs 1 puff normal some orange, some green some lightly speckled/some moderately speckled +/- a lot a lot n/a 4 6.50 -22.00 2018-02-16 13:30 2/16/18 Puffs 2 puff normal mostly orange, few green some lightly speckled +/- a lot some a lot 4 6.50 -22.00 2018-02-16 13:30 2/16/18 tufts 1 tuft normal mostly orange, few green some lightly speckled/few moderately speckled +/- little little n/a 4 6.50 -22.00 2018-02-16 13:30 2/16/18 tufts 2 tuft some normal, some thick orange* none speckled - little little little 5 3.50 -22.00 2018-02-17 10:40 2/17/18 Puff #1 puff normal orange* none speckled - little none little 5 3.50 -22.00 2018-02-17 10:40 2/17/18 Puff #2 puff normal orange majority moderately speckled +/- little some n/a 5 3.50 -22.00 2018-02-17 10:40 2/17/18 Tuft #1 tuft normal orange some lightly speckled/few moderately speckled +/- some some n/a 5 3.50 -22.00 2018-02-17 10:40 2/17/18 Tuft #2 tuft normal orange* few lightly speckled +/- little some n/a 8 -4.00 -22.00 2018-02-20 22:00 2/20 Minis 4 unlabeled/probably puff normal mostly orange, few green some lightly speckled +/- some* some a lot 8 -4.00 -22.00 2018-02-20 22:00 2/20 Minis 3 unlabeled normal mostly orange, few green some lightly speckled +/- some some some 8 -4.00 -22.00 2018-02-20 22:00 2/20 Minis 2 unlabeled/probably puff normal some orange, some green some lightly speckled/some moderately speckled +/- some some a lot 8 -4.00 -22.00 2018-02-20 22:00 2/20 Minis 1 unlabeled normal some orange, some green majority lightly speckled +/- a lot some a lot 11 -5.60 -26.50 2018-02-24 21:00 raft 1 raft some normal, some thick mostly orange, some green some lightly speckled +/- some some n/a 11 -5.60 -26.50 2018-02-24 21:00 raft 2 raft normal mostly orange, some green some moderately speckled +/- some some some 11 -5.60 -26.50 2018-02-24 21:00 puff 1 puff normal some orange, some red, some green few lightly speckled +/- little none a lot 11 -5.60 -26.50 2018-02-24 21:00 puff 2 puff some normal, some thick* some orange, some red, some green few lightly speckled/ some moderately speckled +/- some some a lot 13 0.00 -36.90 2018-02-26 16:17 2/26 1 unlabeled/probably puff normal mostly orange, some green some lightly speckled +/- none none a lot 13 0.00 -36.90 2018-02-26 16:17 2/26 2 unlabeled/probably tuft normal mostly orange, some green none speckled - little none little 13 0.00 -36.90 2018-02-26 16:17 2/26 3 unlableled normal mostly orange, some green few lightly speckled +/- little none n/a 13 0.00 -36.90 2018-02-26 16:17 2/26 4 unlabeled normal mostly orange, some green few lightly speckled +/- little* little n/a 13B (x) 0.00 -36.90 2018-02-27 nd top puff #1 2/27/18 puff normal some orange, some red, some green none speckled - little little little 13B (x) 0.00 -36.90 2018-02-27 nd top puff #2 2/27/18 puff normal mostly orange, some green some lightly speckled +/- little little some/a lot* 13B (x) 0.00 -36.90 2018-02-27 nd bottom puff #1 2/27/18 puff normal some orange, some red, some green none speckled - some little some 13B (x) 0.00 -36.90 2018-02-27 nd bottom puff #2 2/27/18 puff normal some orange, some red, some green some lightly speckled +/- little little n/a 13B (x) 0.00 -36.90 2018-02-27 nd tuft #1 2/27/18 tuft normal some orange, some red, some green few lightly speckled +/- some little some 13B (x) 0.00 -36.90 2018-02-27 nd tuft #2 2/27/18 tuft normal mostly orange, some green none - little some some 14 2.60 -40.00 2018-02-28 18:09 high puff #1 2/28/18 puff normal mostly orange, few green majority moderately speckled +/- some some n/a 14 2.60 -40.00 2018-02-28 18:09 high puff #2 2/28/18 puff normal mostly orange, some green some moderately speckled +/- little none a lot 14 2.60 -40.00 2018-02-28 18:09 low puff #1 2/28/18 puff normal mostly orange, some green some moderately speckled/few lightly speckled +/- some some n/a 14 2.60 -40.00 2018-02-28 18:09 low puff #2 2/28/18 puff normal mostly orange, some green some lightly speckled +/- some some a lot 14 2.60 -40.00 2018-02-28 18:09 tuft #1 2/28/18 tuft some normal, some thick mostly orange, some green some lightly speckled/some moderately speckled +/- some some some 14 2.60 -40.00 2018-02-28 18:09 tuft #2 2/28/18 tuft some normal, some thick mostly orange, some green some lightly speckled/few moderately speckled +/- some some a lot 14 2.60 -40.00 2018-02-28 18:09 3/1/18 ELF puff spread puff normal some orange, some red, some green majority moderately speckled +/- some little little 15 5.00 -44.30 2018-03-02 18:20 3/2/18 tuft 1 tuft normal mostly orange, some green few lightly speckled +/- little little n/a 15 5.00 -44.30 2018-03-02 18:20 3/2/18 ELF tuft 2 tuft normal mostly orange, some green none speckled - some some n/a 15 5.00 -44.30 2018-03-02 18:20 3/2/2018 puff 1 puff normal mostly orange, some green majority moderately speckled +/- some little n/a 15 5.00 -44.30 2018-03-02 18:20 3/2/18 puff 2 puff some normal, some thick mostly orange, some green some lightly speckled/some moderately speckled +/- little some some 15 5.00 -44.30 2018-03-02 18:20 3/3/18 puff 3 puff normal mostly orange, some green some lightly speckled/some moderately speckled* +/- a lot a lot a lot 15 5.00 -44.30 2018-03-02 18:20 3/3/18 puff 4 puff normal some orange, some green majority lightly speckled +/- some some a lot 15 5.00 -44.30 2018-03-02 18:20 3/3/18 raft 3 raft normal mostly orange, some green some moderately speckled +/- some little n/a 15 5.00 -44.30 2018-03-02 18:20 3/3/18 raft 4 raft normal * some moderately speckled +/- a lot a lot a lot 16 7.50 -48.50 2018-03-04 14:30 3/4/18 2:30pm Elf Tuft 2 tuft normal mostly orange, some green none - some some n/a 16 7.50 -48.50 2018-03-04 14:30 3/4/18 2:30pm ELF Puff 1 puff some normal, some thick some orange, some red, some green none - little little n/a 16 7.50 -48.50 2018-03-04 14:30 3/4/18 2:30pm ELF puff 2 puff normal mostly orange, some green majority lightly speckled/some lightly speckled +/- some some some 16 7.50 -48.50 2018-03-04 14:30 3/4/18 2:30pm ELF tuft 1 tuft some normal, some thick mostly orange, few green few lightly speckled +/- some some a lot 17 10.00 -52.70 2018-03-07 nd ELF Puff #1 3/7/18 puff normal some orange, some red, some green some moderately speckled +/- some some a lot 17 10.00 -52.70 2018-03-07 nd ELF Puff and Tuft 3/7/18 puff/tuft some normal, some thick some orange, some red, some green few moderately speckled +/- a lot some a lot 17 10.00 -52.70 2018-03-07 nd ELF Puff #2 3/7/18 puff some normal, some thick* some orange, some red, some green some lightly speckled/some moderately speckled +/- some little n/a 17 10.00 -52.70 2018-03-07 nd ELF Tuft 3/7/18 tuft some normal, some thick some orange, some green majority moderately speckled +/- a lot a lot a lot 17 10.00 -52.70 2018-03-07 nd ELF bottom stuff of (tuse??) 3/7/18 unlabeled/probably puff some normal, some thick some orange, some green majority lightly speckled; few moderately speckled +/- a lot some a lot 17 10.00 -52.70 2018-03-08 10:00 3/8/18 10am puff 1 puff some normal, some thick some orange, some red, some green few lightly speckled +/- some some a lot 17 10.00 -52.70 2018-03-08 10:00 3/8/18 10am puff 2 puff some normal, some thick mostly orange, some green few moderately speckled +/- some some a lot 17 10.00 -52.70 2018-03-08 10:00 3/8/18 10am tuft 1 tuft some normal, some thick mostly orange, some green none - little some some 17 10.00 -52.70 2018-03-08 10:00 3/8/18 10am tuft 2 tuft some normal, some thick *some orange, some green none - a lot little n/a 18 12.80 -57.00 2018-03-09 2:00 3/9/17 2am net puff 1 puff normal some orange* few lightly speckled/few moderately speckled +/- a lot some some 18 12.80 -57.00 2018-03-09 3:00 3/9/18 2am net puff 2 puff some normal, some thick mostly orange, some green few lightly speckled/few moderately speckled* +/- some some a lot 18 12.80 -57.00 2018-03-09 4:00 3/9/17 2am net tuft 1 tuft normal *mostly orange, some green few lightly speckled/few moderately speckled +/- some little a lot 18 12.80 -57.00 2018-03-09 5:00 3/9/18 2am net tuft 2 tuft some normal, some thick mostly orange, some green* few moderately speckled +/- a lot a lot n/a 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm net puff #1 puff some normal, some thick mostly orange, few green majority lightly speckled +/- some some n/a 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm net puff #2 puff some normal, some thick mostly orange, some green none speckled - a lot a lot n/a 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm net tuft #1 tuft some normal, some thick mostly orange, some green few lightly speckled +/- a lot a lot a lot 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm net tuft #2 tuft some normal, some thick some orange, some red, some green few lightly speckled +/- some a lot a lot 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm net raft raft some normal, some thick mostly orange, some green none speckled - a lot a lot a lot 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm puff floaters puff some normal, some thick some orange, some red, some green some lightly speckled +/- some a lot a lot 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm tuft floaters tuft normal mostly orange, few green few lightly speckled +/- a lot a lot some 19 15.50 -61.00 2018-03-10 12:00 3/10/2018 12pm tuft sinkers tuft some normal, some thick brown and gray? some lightly speckled/some moderately speckled +/- a lot a lot a lot 19 15.50 -61.00 2018-03-10 12:00 3/10/18 12pm net puff sinkers puff some normal, some thick orange and green + brown and gray few lightly speckled +/- a lot a lot a lot 19 15.50 -61.00 2018-03-11 8:30 3/11/18 8:30am net puff 1 ELF puff some normal, some thick mostly orange, few green few lightly speckled +/- some little a lot 19 15.50 -61.00 2018-03-11 8:30 3/11/18 8:30am net puff 2 ELF puff some normal, some thick mostly orange, few green* few lightly speckled +/- a lot some a lot 19 15.50 -61.00 2018-03-11 8:30 3/11/18 8:30am net tuft 1 ELF tuft some normal, some thick some orange, some green few lightly speckled +/- some some a lot 19 15.50 -61.00 2018-03-11 8:30 3/11/18 8:30am net tuft 2 ELF tuft some normal, some thick mostly orange, some green none - a lot a lot a lot 20 17.00 -65.40 2018-03-13 5:30 3/13/18 ELF puff 1 puff normal orange* some lightly speckled +/- some some some 20 17.00 -65.40 2018-03-13 5:30 3/13/18 ELF puff 2 puff some normal, some thick mostly orange, few green some lightly speckled +/- a lot some n/a 20 17.00 -65.40 2018-03-13 5:30 3/13/18 ELF tuft 1 tuft normal mostly orange*, few green majority lightly speckled +/- a lot some n/a 20 17.00 -65.40 2018-03-13 5:30 3/13/18 ELF tuft 2 tuft some normal, some thick mostly orange, few green some lightly speckled/few moderately speckled +/- a lot some a lot 20 17.00 -65.40 2018-03-13 5:30 3/13/18 ELF raft 1 raft normal mostly orange, some green * none - a lot some n/a 20 17.00 -65.40 2018-03-13 5:30 3/13/18 ELF raft 2 raft some normal, some thick some orange, some green * some lightly speckled/few moderately speckled +/- a lot a lot a lot